picoquant fcs Search Results


flim fcs capabilities  (PicoQuant inc)
90
PicoQuant inc flim fcs capabilities
Flim Fcs Capabilities, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flim fcs capabilities/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
flim fcs capabilities - by Bioz Stars, 2026-06
90/100 stars
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fcs module  (PicoQuant inc)
90
PicoQuant inc fcs module
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Fcs Module, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fcs module/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
fcs module - by Bioz Stars, 2026-06
90/100 stars
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absolute diffusion coefficients: compilation reference data fcs calibration  (PicoQuant inc)
90
PicoQuant inc absolute diffusion coefficients: compilation reference data fcs calibration
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Absolute Diffusion Coefficients: Compilation Reference Data Fcs Calibration, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/absolute diffusion coefficients: compilation reference data fcs calibration/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
absolute diffusion coefficients: compilation reference data fcs calibration - by Bioz Stars, 2026-06
90/100 stars
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lifetime fluorescence correlation spectroscopy upgrade nikon c1si  (PicoQuant inc)
90
PicoQuant inc lifetime fluorescence correlation spectroscopy upgrade nikon c1si
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Lifetime Fluorescence Correlation Spectroscopy Upgrade Nikon C1si, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lifetime fluorescence correlation spectroscopy upgrade nikon c1si/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
lifetime fluorescence correlation spectroscopy upgrade nikon c1si - by Bioz Stars, 2026-06
90/100 stars
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’s compact flim fcs upgrade kit  (PicoQuant inc)
90
PicoQuant inc ’s compact flim fcs upgrade kit
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
’S Compact Flim Fcs Upgrade Kit, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/’s compact flim fcs upgrade kit/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
’s compact flim fcs upgrade kit - by Bioz Stars, 2026-06
90/100 stars
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fcs/flim module  (PicoQuant inc)
90
PicoQuant inc fcs/flim module
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Fcs/Flim Module, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fcs/flim module/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
fcs/flim module - by Bioz Stars, 2026-06
90/100 stars
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fcs unit  (PicoQuant inc)
90
PicoQuant inc fcs unit
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Fcs Unit, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fcs unit/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
fcs unit - by Bioz Stars, 2026-06
90/100 stars
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absolute diffusion coefficients: compilation reference data fcs calibration, rev 1  (PicoQuant inc)
90
PicoQuant inc absolute diffusion coefficients: compilation reference data fcs calibration, rev 1
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Absolute Diffusion Coefficients: Compilation Reference Data Fcs Calibration, Rev 1, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/absolute diffusion coefficients: compilation reference data fcs calibration, rev 1/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
absolute diffusion coefficients: compilation reference data fcs calibration, rev 1 - by Bioz Stars, 2026-06
90/100 stars
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picoquant flim fcs system  (Protein Sciences Inc)
86
Protein Sciences Inc picoquant flim fcs system
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Picoquant Flim Fcs System, supplied by Protein Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/picoquant flim fcs system/product/Protein Sciences Inc
Average 86 stars, based on 1 article reviews
picoquant flim fcs system - by Bioz Stars, 2026-06
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Image Search Results


Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . Fluorescent microscope images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for FCS studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.

Journal: FEBS Open Bio

Article Title: Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences

doi: 10.1002/2211-5463.12432

Figure Lengend Snippet: Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . Fluorescent microscope images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for FCS studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.

Article Snippet: The live‐cell confocal imaging and FCS measurements were carried out using FV1000 inverted epifluorescence microscope (Olympus, Hamburg) equipped with FCS module (PicoQuant GmbH, Berlin) and an UplanSApo 60 × water immersion objective lens (NA 1.2).

Techniques: Confocal Laser Scanning Microscopy, Expressing, Transfection, Microscopy, Fluorescence, Mutagenesis, Construct, Amplification